Fred Sanger: sequencing pioneer

Fred Sanger: a memorial tribute.

The first sequence. Fred Sanger and insulin.

FRED Sanger is an amazingly modest man, and his into the Laboratory of Molecular Biology), took me on as a research student. Vernon had just shown that sickleown retrospective, written after he retired, a delightful prefatory chapter for the Annual Reviews of cell hemoglobin differed from normal hemoglobin by a single amino acid substitution, the first characterizaBiochemistry, is called “Seque...

Would Fred Sanger Get Funded Today?

Fred Sanger developed technologies that won him two Nobel Prizes and revolutionized biological research. Yet, in spite of this record, the question has been raised as to whether, in the current scientific climate, he might be unsuccessful in obtaining a grant because of a productivity that would be viewed as too limited. In imagining how a National Institutes of Health study section today might...

Part One Sanger DNA Sequencing

Pyrosequencing-An Alternative to Traditional Sanger Sequencing

Problem statement: Pyrosequencing has the potential to rapidly and reliably sequence DNA taking advantages over traditional Sanger di-deoxy sequencing approach. Approach: A comprehensive review of the literature on the principles, applications, challenges and prospects of pyrosequencing was performed. Results: Pyrosequencing was a DNA sequencing technology based on the sequencing-by-synthesis p...